Zur Farbreaktion der phenolischen Steroide mit Überchlorsäure
- 1 January 1954
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 298 (Jahresband) , 268-271
- https://doi.org/10.1515/bchm2.1954.298.1.268
Abstract
The reaction involves heating the steroids in chloroform with a reagent (containing 0.1 ml 70% perchloric acid, 3 ml absolute alcohol and 7 ml chloroform) and salicylic acid, for 5 minutes on a boiling water bath. The residue is dissolved in a phenol-chloroform mixture, and aliquots added to ether with and without peroxide. The former gives the blank, the latter the experimental value. A calibration curve for 0.40 ug is presented. The extraction of the steroids from urine is described. The recovery of steroids added to urine is only 50%.Keywords
This publication has 2 references indexed in Scilit:
- Die Eliminierung störender Chromogene bei der Bestimmung der 17-Ketosteroide mit m-DinitrobenzolHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1952
- The colorimetric estimation of the oestrogenic hormonesBiochemical Journal, 1938