Comparative Oligonucleotide Analysis of Exogenous and Endogenous Primate Type C Viruses

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Abstract
RNA of representative viruses of the exogenous simian sarcoma virus-gibbon ape lymphosarcoma virus (SiSV/GALV) and baboon endogenous virus (BaEV) classes of subhuman primate type C viruses were compared and related to HEL-12 virus and isolate derived from human embryonic lung cells. The extent of sequence identity between different viral RNA preparations was determined by comparison of fingerprint patterns obtained after electrophoretic separation of RNase T1-resistant oligonucleotides. HEL-12 viral RNA and simian sarcoma-simian associated virus [SiSV(SSAV)] RNA apparently share 90-95% of the large oligonucleotides. From 5-10% of virus-specific oligonucleotides were detected in each of several virus preparations examined and their occurrence was independent of the cell line on which the virus was propagated. HEL-12 virus and GALV-SF have 50% unique oligonucleotides in common. These are the same oligonucleotides that are shared between GALV-SF and SiSV(SSAV) RNA. Two BaEV isolates, M7 and BILN, and RD 114, a BaEV-related endogenous virus of cats, each display distinguishable oligonucleotide patterns. Large oligonucleotides characteristic for these 3 endogenous virus isolates were not detected in the fingerprints of HEL-12 virus, SiSV(SSAV) and GALV-SF.