DECLINE IN ASSAYABLE PROGESTERONE IN BOVINE PLASMA - EFFECT OF TIME, TEMPERATURE, ANTI-COAGULANT, AND PRESENCE OF BLOOD-CELLS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (3) , 521-522
Abstract
Jugular vein blood samples collected from 5 Holstein-Friesian cows during the luteal phase of the estrous cycle were incubated at 4, 22 and 37.degree. C for 0, 0.5, 1, 2, 4, 6, 8, 12 and 24 h. Blood samples were centrifuged after each incubation period, and plasma was frozen for later progesterone assay. Mean progesterone concentration in blood samples incubated at 4, 22 and 37.degree. C decreased from 5.7 ng/ml at 0 h to minimum concentrations of 3.5 ng/ml at 12 h, 0.9 ng/ml at 12 h and 1.0 ng/ml at 8 h, respectively. The 1st progesterone concentration which was significantly different from the initial value (0 h) was at the 8 h incubation period for blood samples incubated at 4.degree. C and at the 1 h incubation period for blood samples incubated at 22 and 37.degree. C. Progesterone concentration decreased similarly when blood samples were collected into heparinized tubes or EDTA tubes and incubated at 22.degree. C for 0, 6 and 12 h. Incubating harvested plasma or serum for 0 or 6 h at 37.degree. C had no effect on progesterone concentrations (7.9 and 8.2 ng/ml for plasma and 6.9 and 6.6 ng/ml for serum).

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