Neurotrophic Effects of Epidermal Growth Factor on Cultured Brain Neurons Are Blocked by Protein Kinase Inhibitors
Open Access
- 1 January 1992
- journal article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 59 (2) , 259-261
- https://doi.org/10.1254/jjp.59.259
Abstract
The influences of protein kinase inhibitors, K-252a and staurosporine, on the neurotrophic effects of epidermal growth factor (EGF) were investigated in dissociated cell cultures of the hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml EGF enhanced the survival of cultured neurons of both brain regions. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival-promoting effects of EGF in a concentration-dependent manner. These results suggest that activation of protein kinase(s) is involved in the neurotrophic effects of EGF.Keywords
This publication has 10 references indexed in Scilit:
- Basic Fibroblast Growth Factor and Epidermal Growth Factor Promote Survival of Primary Cultured Cerebellar Neurons from Neonatal Rats.The Japanese Journal of Pharmacology, 1991
- Involvement of Protein Kinase Activation in Neurotrophic Effects of Basic Fibroblast Growth Factor in Cultured Brain Neurons.The Japanese Journal of Pharmacology, 1991
- A Comparison of Neurotrophic Effects of Epidermal Growth Factor and Basic Fibroblast Growth Factor in Primary Cultured Neurons from Various Regions of Fetal Rat BrainThe Japanese Journal of Pharmacology, 1990
- Effects of Recombinant Human Basic Fibroblast Growth Factor and Its Modified Protein CS23 on Survival of Primary Cultured Neurons from Various Regions of Fetal Rat BrainThe Japanese Journal of Pharmacology, 1990
- Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinasesTrends in Pharmacological Sciences, 1989
- Trophic Stimulation of Cultured Neurons from Neonatal Rat Brain by Epidermal Growth FactorScience, 1987
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- K-252a, a potent inhibitor of protein kinase C from microbial origin.The Journal of Antibiotics, 1986
- Epidermal Growth Factor Immunoreactive Material in the Central Nervous System: Location and DevelopmentScience, 1984
- Epidermal Growth FactorAnnual Review of Biochemistry, 1979