The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of Synechocystis PCC6803
Open Access
- 29 December 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 487 (2) , 272-276
- https://doi.org/10.1016/s0014-5793(00)02369-3
Abstract
The physiological function of the type 1 NAD(P)H dehydrogenase (Ndh‐1) of Synechocystis sp. PCC6803 has been investigated by inactivating the gene ndhH encoding a subunit of the complex. Molecular analysis of independent transformants revealed that all clones were heteroploid, containing both wild‐type and mutant ndhH copies, whatever the metabolic conditions used during genome segregation, including high CO2 concentration. By replacing the chromosomal copy of the ndhH gene by a plasmidial copy under the control of a temperature‐controlled promoter, we induce a conditional phenotype, growth being only possible at high temperature. This clearly shows for the first time that an ndh gene is indispensable to the survival of Synechocystis sp. PCC6803.Keywords
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