Effect of Aging on Survival and Pathogenicity of Eimeria acervulina and Eimeria tenella

Abstract
After 6-8 mo. of storage, cultures of sporulated E. acervulina and E. tenella oocysts had a marked drop in the number of sporocysts that survived grinding, sporozoites that survived after excystation and sporozoites that penetrated chick kidney cells in vitro. The rate of excystation was unaffected by storage of up to 1 yr. In vivo pathogenicity, based on weight gain, lesion score and plasma pigment, declined after 6 mo. of oocyst storage. The reduction in pathogenicity in vivo could be compensated for by adjusting the inoculation dosage to reflect the loss of infectivity seen in the in vitro test.

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