Serotonin Induces EPSCs Preferentially in Layer V Pyramidal Neurons of the Frontal Cortex in the Rat
Open Access
- 1 October 2000
- journal article
- research article
- Published by Oxford University Press (OUP) in Cerebral Cortex
- Vol. 10 (10) , 974-980
- https://doi.org/10.1093/cercor/10.10.974
Abstract
The effect of serotonin (5-HT) on the release of glutamate was examined in pyramidal cells in layers II–VI of the frontal cortex. The intracellular recording electrode contained 1% biocytin so the neurons could later be visualized with an avidin-biotin peroxidase method. Pyramidal cells in layer V of the frontal cortex showed the greatest 5-HT-induced increase in both the frequency and amplitude of ‘spontaneous’ (non-electrically evoked) excitatory post-synaptic currents (EPSCs). A small proportion of neurons in layer II/III showed an increase in EPSC frequency, whereas cells in layer VI showed no significant change in either EPSC frequency or amplitude. The physiological response to 5-HT mirrors the high density of 5-HT2A receptors in layer V, as well as the pattern of thalamic projections in frontal cortex. The specific induction of EPSCs in layer V neurons suggests that 5-HT preferentially modulates the output neurons of the frontal cortex.Keywords
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