RAPID VIRUS DIAGNOSIS BY ELECTROSYNERESIS

  • 1 January 1978
    • journal article
    • research article
    • Vol. A129  (4) , 545-552
Abstract
Electrosyneresis (counter-current immunoelectrophoresis) detected the presence of 7 coxsackieviruses and 1 poliovirus in 70 specimens [human] of stools. Identical results were obtained by inoculation of mice and tissue cultures. First screening of specimens was undertaken with a human standard gammaglobulin; only specimens precipitating with this reagent contained viral antigens. Hence, especially in children, all negative stools could be excluded from subsequent tests with antiserum pools and monospecific antisera. Electrosyneresis is a specific method: every type of coxsackievirus and poliovirus types react only with the corresponding monospecific antisera. Electrosyneresis appears to be a sensitive procedure: viral antigens corresponding to 104 TCID50[median tissue culture infective dose]/ml can be detected.

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