Optimization of a Human Papillomavirus-Specific Enzyme-Linked Immunosorbent Assay
Open Access
- 1 May 2002
- journal article
- Published by American Society for Microbiology in Clinical and Vaccine Immunology
- Vol. 9 (3) , 577-82
- https://doi.org/10.1128/cdli.9.3.577-582.2002
Abstract
A strategy was developed for the control, standardization, and critical evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of human papillomavirus-specific immunoglobulin G in human sera. Control human sera, polyclonal animal sera, and monoclonal antibodies were used to establish optimal assay parameters, including antigen coating, serum dilutions, and criteria for daily reproducibility, monitoring, and rejection of assays. Three evaluation techniques were used in parallel to define an optimal cutoff absorbance value that yields greater than 93% sensitivity and 98.5% specificity in the assay's ability to discriminate positive and negative control sera. This strategy provides an optimal method by which to determine cutoff absorbance values for ELISA.Keywords
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