Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.
- 1 May 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (5) , 2651-2655
- https://doi.org/10.1073/pnas.77.5.2651
Abstract
When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRi, a DNA fragment of 14.0 kilobase [kb] can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kb EcoRi DNA fragment was cloned in bacteriophage .lambda.. The sequence organization of a recombinant clone, .lambda. .cntdot. MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping and EM. .lambda. .cntdot. MTX-1 contains an uninterrupted stretch of 1.0 kb similar to that found in the M-MSV genome.This publication has 21 references indexed in Scilit:
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