In vitro biosynthesis of the human cell surface receptor for transferrin
- 25 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (2) , 259-264
- https://doi.org/10.1016/0014-5793(83)80591-2
Abstract
The human cell surface receptor for transferrin is a transmembrane phosphoglycoprotein composed of two disulphide linked and apparently identical subunits of M r 90 000. Using an affinity purified, polyclonal rabbit antibody, we have studied the in vitro biosynthesis of this receptor. The primary translation product, synthesised in a rabbit reticulocyte lysate programmed with human placental RNA, appears to have the same M r (78 000) as the unglycosylated molecule immunoprecipitated from tunicamycin‐treated cells. In the presence of a dog pancreatic microsomal system the cell free system accurately reproduces the glycosylation and the asymmetric transmembrane integrationKeywords
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