Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome.
- 15 July 1993
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (14) , 6415-6419
- https://doi.org/10.1073/pnas.90.14.6415
Abstract
We establish that linker histones H1 and H5 bind preferentially to a Xenopus borealis somatic 5S RNA gene associated with an octamer of core histones rather than to naked 5S DNA. This preferential binding requires free linker DNA to either side of the nucleosome core. Incorporation of a single linker histone molecule into the nucleosome protects an additional 20 bp of linker DNA from micrococcal nuclease digestion. This additional DNA is asymmetrically distributed with respect to the nucleosome core. Incorporation of linker histones causes no change to the cleavage of DNA in the nucleosome by hydroxyl radical or DNase I.Keywords
This publication has 32 references indexed in Scilit:
- Chromatosome positioning on assembled long chromatin: Linker histones affect nucleosome placement on 5 S rDNAJournal of Molecular Biology, 1991
- Nucleosome Positioning: Occurrence, Mechanisms, and Functional ConsequencesProgress in Nucleic Acid Research and Molecular Biology, 1991
- Direct detection of linker DNA bending in defined-length oligomers of chromatin.Proceedings of the National Academy of Sciences, 1990
- The structure of DNA in a nucleosome.Proceedings of the National Academy of Sciences, 1990
- DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.Proceedings of the National Academy of Sciences, 1990
- Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1Biochemistry, 1989
- [33] Hydroxyl radical footprinting: A high-resolution method for mapping protein-DNA contactsPublished by Elsevier ,1987
- A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting itCell, 1986
- Salt-dependent co-operative interaction of histone H1 with linear DNAJournal of Molecular Biology, 1986
- Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: A model system for study of higher order structureCell, 1985