Variations in Immunogenicity of Disrupted Cells Prepared From an Adenovirus-7 Hamster Tumor Cell Line2

Publisher Website
Google Scholar
Abstract
Adenovirus-7 hamster tumor cells propagated in cell culture varied markedly with respect to capability to immunize against homologous transplant tumor after treatment with formaldehyde or disruption by various mechanical means. Protective vaccines employed cells for vaccine preparation or for challenge that were at passage levels comparable to those of the nonprotective vaccines. The protective vaccines were made with highly oncogenic cells, while the cells used for nonprotective vaccines varied, ranging from weakly to highly oncogenic. The basis for the differences in immunogenicity of the vaccines is not known. However, all protective vaccines were prepared from the descendants of a single vial of frozen parent-cell seed stock, while the nonprotective vaccines came from descendants of other frozen vials of the same parent seed. Selection of qualitatively different progeny from the parent cell population appeared to have taken place. Possible difference in the kind of immune response e.g., cell-mediated compared with humoral antibody (tumor-enhancing), is being investigated.

This publication has 0 references indexed in Scilit: