PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION
Open Access
- 1 December 1974
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 22 (12) , 1084-1091
- https://doi.org/10.1177/22.12.1084
Abstract
A new method of conjugating horseradish peroxidase with proteins was developed. The carbohydrate moiety of fluorodinitrobenzene-blocked peroxidase was oxidized with sodium periodate to form aldehyde groups. The peroxidase-aldehyde was then bound to free amino groups of proteins unidirectionally at high efficiencies. Peroxidase-labeled immunoglobulin retained its immunologic as well as enzymatic activities.Keywords
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