In vitro studies on bile acid deconjugation and lipolysis inhibition byGiardia lamblia

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Abstract
In vitro studies were conducted to determine whetherGiardia lamblia can deconjugate bile acids or inhibit lipolysis. CulturedG. lamblia trophozoites failed to deconjugate either glycine- or taurine-conjugated cholic acid, deoxycholic acid, or chenodeoxycholic acid. However, sonicated trophozoites significantly inhibited the hydrolysis of tributyrylglycerol by porcine pancreatic lipase. This inhibition was not dependent upon the presence of bile acids but was dependent upon the concentration ofG. lamblia sonicate. Heating the sonicate for 20 min at 60° C abolished the inhibitory effect; dialysis of the sonicate did not affect inhibition. We conclude that culturedG. lamblia trophozoites do not deconjugate bile acids and that sonicatedG. lamblia inhibit lipolysisin vitro.