Determination of Thymidine in Serum Used for Cell Culture Media
- 1 January 1978
- journal article
- research article
- Published by S. Karger AG in Pathobiology
- Vol. 46 (1-2) , 1-10
- https://doi.org/10.1159/000162879
Abstract
Thymidine concentrations in serum used for cell culture media were determined with an assay based on isotope dilution. In this assay, incorporation of (3H)-thymidine into DNA of cultured cells was measured in the presence of 5 and 20% serum as a function of the concentration of unlabeled thymidine added to the medium. In three batches of horse serum, thymidine concentrations were 0-0.17 µM, while in fetal calf serum values of 0.75-2.1 µMwere obtained. Dialysis of serum resulted in a reduction of thymidine levels by factors of at least 10.This publication has 1 reference indexed in Scilit:
- The requirement of mammalian cell cultures for serum proteins. Growth-promoting activity of pepsin-digested serum albumin in different mediaBiochimica et Biophysica Acta (BBA) - Protein Structure, 1967