Alkylation of Phosphates and Stability of Phosphate Triesters in DNA
- 1 November 1972
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 31 (1) , 103-111
- https://doi.org/10.1111/j.1432-1033.1972.tb02506.x
Abstract
A method is presented to measure the alkylation of phosphates in DNA after a treatment with an alkylating agent. Using this method, we have shown that phosphate alkylation represents 15% of total alkylation when DNA is alkylated with ethyl methanesulfonate and only 1% of total alkylation when DNA is alkylated with methyl methanesulfonate. Experiments are also presented which show that phosphate triesters resulting from the alkylation of DNA by ethyl methanesulfonate are very stable, most of them remaining intact after heating at 100°C for 90 min at pH 7.0.Keywords
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