A myeloma hybrid producing antibody specific for an allotypic determinant on “IgD‐like” molecules of the mouse

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Abstract
A hybrid cell line was produced by fusing a mouse myeloma line with spleen cells from BALB/c mice immunized with B10 cells. The hybrid line grew in tissue culture and in syngeneic mice and produced IgM antibody specific for “IgD‐like” molecules of mice with the Igb haplotype. The concentration of monoclonal antibody in the serum of tumor‐bearing animals reached about 2 mg/ml and gave cytotoxic titers of up to 1:800 000. The derivation of the line, some properties of the antibody secreted and the nature of its antigenic target aredescribed.