Thymine glycol and thymidine glycol in human and rat urine: a possible assay for oxidative DNA damage.
- 1 September 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (18) , 5633-5637
- https://doi.org/10.1073/pnas.81.18.5633
Abstract
Thymine glycol is a DNA damage product ionizing radiation and other oxidative mutagens. To find a noninvasive assay for oxidative DNA damage in individuals, a HPLC [high performance liquid chromatography] assay was developed for free thymine glycol and thymidine glycol in urine. Humans excrete .apprx. 32 nmol of the 2 glycols/day. Rats, which have a higher specific metabolic rate and shorter life span, excrete .apprx. 15 times more thymine glycol plus thymidine glycol/kg of body wt than do humans. Thymine glycol and thymidine glycol are likely to be derived from repair of oxidized DNA, rather than from alternative sources such as the diet or bacterial flora. This noninvasive assay of DNA oxidation products may allow the direct testing of current theories which relate oxidative metabolism to the processes of aging and cancer in man.Keywords
This publication has 23 references indexed in Scilit:
- Hydroxymethyluracil DNA glycosylase in mammalian cells.Proceedings of the National Academy of Sciences, 1984
- Dietary Carcinogens and AnticarcinogensScience, 1983
- Mammalian DNA‐repair endonuclease acts only on supercoiled DNAFEBS Letters, 1982
- DNA Repair EnzymesAnnual Review of Biochemistry, 1982
- Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis.Proceedings of the National Academy of Sciences, 1981
- Purification and Properties of a Mouse‐Cell DNA‐Repair Endonuclease, which Recognizes Lesions in DNA Induced by Ultraviolet Light, Depurination, γ‐Rays, and OsO4 TreatmentEuropean Journal of Biochemistry, 1980
- DNA N-glycosylases and UV repairNature, 1980
- Urinary excretion of methylated purines and 1-methyl-nicotinamide following administration of methylating carcinogensChemico-Biological Interactions, 1980
- Selective excision of gamma ray damaged thymine from the DNA of cultured mammalian cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Determination of Base Sequence in Nucleic Acids with the Electron Microscope. V. The Thymine-Specific Reactions of Osmium Tetroxide with Deoxyribonucleic Acid and Its Components*Biochemistry, 1966