Microbial conversion of steffimycin and steffimycin B to 10-dihydrosteffimycin and 10-dihydrosteffimycin B.

Abstract
Steffimycin (1) and steffimycin B (2) are reduced at the C-10 carbonyl by Actinoplanes utahensis, UC-5885 and Chaetomium sp., UC-4634, respectively. Using cell-free extracts of the latter organism, the optimum conversion time, pH and enzyme concentration were determined for the conversion of 2 and 4. The biochemical conversion of 2 was TPNH linked.