In vivo and In vitro Studies of Labeled Antibodies Against Rat Kidney and Walker Carcinoma.

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Abstract
An in vitro method is described for demonstrating binding by rat organ homogenates of I131 labeled antibodies produced by rabbits immunized with particular rat organs. By this test anti-kidney antibodies show specificity for kidney compared with other rat tissues, and anti-Walker rat carcinoma 256 antibodies specificity for this tumor tissue. These data are compared with I131 distributions found after injection of these antibodies by various routes into living rats. Results suggest that nearly all of these antibody molecules are also general anti-rat antibodies in the sense that they are bound by components of other rat tissues. Such factors as relative circulation rates, blood vessel permeability to proteins, intracellular or extracellular location of antigens, and relative organ specificity of antibodies as shown by in vitro experiments consequently are probably important in determining localization of such antibodies in animal injection experiments.