The Role of the Splenic Macrophage in Antihemophilic Factor (Factor VIII) Synthesis
- 1 September 1971
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Surgery
- Vol. 103 (3) , 398-401
- https://doi.org/10.1001/archsurg.1971.01350090080017
Abstract
Rabbit spleen cells cultured in medium 199 with 20% fetal bovine serum or 20% autologous serum showed no measurable supernatant factor VIII (AHF) activity. In acellular control tissue culture vials containing medium 199, 20% horse serum, and 20% autologous plasma, activity averaged 29% at the beginning of incubation and 10% after 24 hours. In all instances, activity decreased to <1% by the end of the second day. In comparison, when initially heterogeneous rabbit spleen cell populations were cultured in medium 199, 20% horse serum, and 20% autologous plasma, a ralatively homogeneous macrophage culture was achieved by the third day, and measurable AHF levels were maintained for as long as nine days. Persistent AHF levels found in cultures but not in controls are interpreted as due to de novo synthesis of AHF by macrophages.This publication has 3 references indexed in Scilit:
- Experimental Transplantation of the Spleen for Classical Hemophilia A Rationales and Long-Term ResultsPublished by S. Karger AG ,1969
- Factors affecting the dye exclusion test for cell viabilityExperimental Cell Research, 1964
- Ethionine-Induced Depression of Plasma Antihemophilic Globulin in the RatExperimental Biology and Medicine, 1954