HIGH-FREQUENCY VARIATION AND POPULATION DRIFT IN A NEWLY TRANSFORMED CLONE OF BALB/3T3 CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (11) , 5242-5248
Abstract
During repeated passage of BALB/3T3 cells and testing for anchorage-independent growth, a single transformed clone was isolated from agar and 5 subclones were derived from it. These subclones differed from one another in morphology on a solid substratum, efficiency and size of colony formation in agar and rate of tumor formation in nude mice. With weekly passage over a period of 6 mo., the differences in morphology and growth in agar gradually decreased. The sublcone which produced the fastest-growing tumors in nude mice after 4 wk of culture produced the slowest-growing tumors after 18 wk and a change in the opposite direction was made by another subclone. There was no difference among the subclones in growth rate on plastic. The distribution of chromosome numbers was heterogenous but overlapping in all the primary subclones at 16 and 24 wk, with no statistically significant difference in the mean number of chromosomes per subclone. An extremely high degree of variation must have occurred to produce the multiple differences between the subclone and the same type of variation could have been responsible for the subsequent changes with repeated passage. The high frequency and graded nature of the changes and the concurrent involvement of several traits suggest an epigenetic basis for the variation.