A Rapid Approach to Selecting the Best Experimental Conditions for High-Speed Liquid Column Chromatography Part II--Estimating Column Length, Operating Pressure and Separation Time for Some Required Sample Resolution
- 1 June 1972
- journal article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 10 (6) , 369-379
- https://doi.org/10.1093/chromsci/10.6.369
Abstract
A simple procedure is described which allows the rapid selection of experimental conditions for achieving some required change in resolution in liquid column chromatography. In conjunction with the procedure outlined in Part I, an initial chromatogram can be evaluated in terms of resolution, a decision made on the necessary change in resolution, and experimental conditions predicted for this required resolution. No calculations are required. The entire procedure from inspection of the initial chromatogram to the final prediction of conditions takes about one minute.Keywords
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