Effect of Diltiazem on in vitro Rabbit Bladder Function

Publisher Website
Google Scholar
Abstract
The relationship between extracellular calcium and urinary bladder function was investigated by studying the effect of the specific calcium antagonist diltiazem on the functional ability of the in vitro whole rabbit urinary bladder to empty in response to pharmacological stimulation. The bladder was found to require an extracellular calcium concentration of 4.5 × 10–4M to elicit near complete cholinergic-mediated emptying. Diltiazem (1 × 10–6–1 × 10–4M) inhibition of bladder function was competitively antagonized by increasing the extracellular calcium concentration (0.45 × 10–4–3.6 × 10–4M). In the absence of diltiazem, alterations in the extracellular calcium concentration between 0.45 × 10–4 and 3.6 × 10–4M had no significant effects on bladder response to bethanechol.