Regiodependent Luminescence Quenching of BiotinylatedN-Sulfonyl-acridinium-9-carboxamides by Avidin
- 16 September 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 5 (21) , 3779-3782
- https://doi.org/10.1021/ol035323p
Abstract
Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.Keywords
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