Petri Dish Concavity—A Potential Source of Error in Antibiotic Assay and Agar Diffusion Antibiotic Susceptibility Tests

Abstract
Concave deformities of the bottom of Petri dishes are a potential source of error for disk agar diffusion antibiotic assay and susceptibility procedures. This is due to differences in agar depth between the central and peripheral areas of the Petri dishes. We have observed significant concave deformities in both empty and commercially filled 150-mm plastic Petri dishes. For this reason we recommend that inspections for Petri dish deformity be incorporated into microbiology laboratory quality control programs.

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