Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine
- 1 May 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 20 (5) , 606-607
- https://doi.org/10.1093/clinchem/20.5.606
Abstract
The hydrogen peroxide produced from glucose by glucose oxidase action at pH 7 is determined by the peroxidase oxidative coupling of N,N-diethylaniline with 4-amino-antipyrine. A 20-µl sample of plasma or serum is reacted at room temperature for 10 min. The sensitivity of the method is such that 60 µg of glucose (300 mg/dl) in a final volume of 5 ml gives an absorbance of 0.8 at 553 nm with a 1-cm cell. Absorbances are linearly related to glucose concentrations as high as 10.00 g/liter.Keywords
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