Spatial relationship of nebulin relative to other myofibrillar proteins during myogenesis in embryonic chick skeletal muscle cellsin vitro

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Abstract
The developmental expression of nebulin was studied in embryonic chick skeletal muscle cellsin vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin andα-actinin but not myosin and connectin (titin). Striated patterns of nebulin (‘singlets’) in myofibrils appeared simultaneously with those ofα-actinin (Z-bands), myosin (A-bands) and connectin (‘doublets’), but earlier than those of actin. After actin striations were formed as myofibrils matured, each nebulin band started to exhibit ‘droplets’. The delayed development of nebulin compared to the I-Z-I brush formation and the myofibril maturation seems to indicate that this giant myofibrillar protein is unnecessary for both the initial (formation of I-Z-I-like structures) and the subsequent (regular alignment of myofibrils) phases of myofibrillogenesis.