Alkaline extraction of toxin from spores of the mosquito pathogen, Bacillus sphaericus strain 1593

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Abstract
Toxin was extracted from spores of the mosquito pathogen B. sphaericus strain 1593 using 0.05 M NaOH. The MW of this toxin was 35,000-54,000. Toxic activity of this extract was resistant to a variety of enzymes including subtilisin, but was degraded by pronase. Antiserum produced to 1593 spore toxin neutralized spore toxin and cytoplasmic toxin activity, but did not react with B. thuringiensis var. israelensis crystal toxin, nor did B. t. israelensis toxin antiserum react with B. sphaericus toxin. Crystallike parasporal inclusions accompanying the B. sphaericus 1593 spores were removed by NaOH extraction.