Studies on Lysostaphin

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Abstract
The bacteriolytic agent lysostaphin has been isolated from the culture supernatant of Staphylococcus epidermidis, NRRL B‐2628 (K‐6‐W1) on the ion‐exchange resin Amberlite IRC‐50. A salt gradient released three enzyme specificities which were further purified to apparent homogeneity. These enzymes, belonging to three different classes, were endo‐β‐N‐ acetylglucosaminidase, endopeptidase (glycinase) and N‐acetylmuramyl‐l‐alanine amidase. In contrast to the first two enzymes, the third one was not found to be bacteriolytic per se.