Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (5) , 1539-1552
- https://doi.org/10.1093/nar/4.5.1539
Abstract
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.Keywords
This publication has 21 references indexed in Scilit:
- Methylmercury as a reversible denaturing agent for agarose gel electrophoresisAnalytical Biochemistry, 1976
- Buoyant density sedimentation of macromolecules in sodium iothalamate density gradientsJournal of Molecular Biology, 1975
- In vitro iodination of DNA. Maximizing iodination while minimizing degradation. Use of buoyant density shifts for DNA-DNA hybrid isolationBiochemistry, 1974
- Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: VII. Mapping the ribosomal RNA genes of plasmid F14Journal of Molecular Biology, 1974
- [29] Analysis of repeating DNA sequences by reassociationPublished by Elsevier ,1974
- Renaturation of bacteriophage φX174 DNA-RNA hybrid: RNA length effect and nucleation rate constantJournal of Molecular Biology, 1973
- Use of formamide in nucleic acid reassociationBiochemistry, 1972
- Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeastJournal of Molecular Biology, 1972
- Kinetic complexity of RNA moleculesJournal of Molecular Biology, 1972
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962