Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
Open Access
- 1 July 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 36 (1) , 59-72
- https://doi.org/10.1099/0022-1317-36-1-59
Abstract
Summary Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.This publication has 5 references indexed in Scilit:
- Polypeptide Phosphorylation in Adenovirus-Infected CellsJournal of General Virology, 1977
- Correlation between tumor induction and the large external transformation sensitive protein on the cell surface.Proceedings of the National Academy of Sciences, 1976
- Adenovirus transcriptionJournal of Molecular Biology, 1976
- Viral nucleic acid sequences in transformed cellsVirology, 1976
- Rapidly labeled HeLa cell nuclear RNAJournal of Molecular Biology, 1966