Influence of Cultivation Media on Genetic Regulatory Patterns in Borrelia burgdorferi
Open Access
- 1 June 2001
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (6) , 4159-4163
- https://doi.org/10.1128/iai.69.6.4159-4163.2001
Abstract
Barbour-Stoenner-Kelly II (BSKII) medium and BSKH medium both are routinely used for the cultivation of Borrelia burgdorferi . However, heretofore there have been no studies to compare how these two media affect gene expression patterns in virulent B. burgdorferi . In the present study, we found that some B. burgdorferi strain 297 genes (e.g., ospA , mlp-7A , mlp-8 , p22 , and lp6.6 ) that typically are regulated by temperature or pH displayed their predicted pattern of expression when B. burgdorferi was cultivated in BSKH medium; this was not true when spirochetes were cultivated in conventional BSKII medium. The results suggest that BSKH medium is superior to BSKII medium for gene expression studies with B. burgdorferi .Keywords
This publication has 18 references indexed in Scilit:
- Identification of 11 pH-Regulated Genes in Borrelia burgdorferi Localizing to Linear PlasmidsInfection and Immunity, 2000
- Expression and Immunological Analysis of the Plasmid-BornemlpGenes ofBorrelia burgdorferiStrain B31Infection and Immunity, 2000
- Decorin-Binding Protein A (DbpA) of Borrelia burgdorferi Is Not Protective When Immunized Mice Are Challenged via Tick Infestation and Correlates with the Lack of DbpA Expression by B. burgdorferi in TicksInfection and Immunity, 2000
- Molecular and Evolutionary Characterization of the cp32/18 Family of Supercoiled Plasmids in Borrelia burgdorferi 297Infection and Immunity, 2000
- Serum-starvation-induced changes in protein synthesis and morphology of Borrelia burgdorferiMicrobiology, 2000
- A new animal model for studying Lyme disease spirochetes in a mammalian host-adapted state.Journal of Clinical Investigation, 1998
- Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine.The Journal of Experimental Medicine, 1996
- Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease.The Journal of Experimental Medicine, 1996
- Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologueMolecular Microbiology, 1995
- Induction of an outer surface protein on Borrelia burgdorferi during tick feeding.Proceedings of the National Academy of Sciences, 1995