RESPONSE OF SPLEEN CELLS FROM IMMUNIZED RABBITS TO CROSS-REACTING ANTIGENS IN IN VITRO SYSTEM

  • 1 January 1964
    • journal article
    • research article
    • Vol. 7  (6) , 665-+
Abstract
Rabbits were immunized with bovine (BSA) or human (HSA) serum albumin. Spleen cell suspensions were prepared and incubated with the homologous or cross-reacting albumins. The antigen-dependent stimulation of the rate of DNA synthesis was used as a measure of the antigenic activity of each of the albumin preparations. The response was proportional to the logarithm of the antigen concentration for both the homologous and the cross-reacting albumins and the slopes of each curve were approximately the same. The cross-reacting albumins were less stimulatory than the homologous antigen. The pattern of their relative activities in this system followed the same pattern shown in their serological cross-reactions.