Application of Chromatography to Segregation Studies of the Agent of Chicken Tumor I (Rous Sarcoma Virus)

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Abstract
A partially purified chicken tumor extract was prepared in 0.9% saline ([long dash]0.8 log molar) and equal amts. were adsorbed on identical microcolumns, consisting of 0.5 g. of Celite as adsorbent. Seven columns with the adsorbed agent were developed with 10 ml. of a Na-Cl soln. ranging in log molarity from[long dash]2 through[long dash]5 and ending with dist. water. The elute from each column was analyzed for N and tested in a balanced quantitative bioassay for tumor agent. Eluants with less than 0.001 M salt conc. released more agent per unit vol. than was present in the starting extract. In contrast to the regional conc. of the virus in the top of the column, N unassociated with virus activity was distributed throughout the column and in the filtrate. The agent particulates associated with chicken tumor I were thus segregated from some of the nonvirus N components of the previously purified extract. The method of chromatographic adsorption may be adaptable not only to the separation of pathological particulates but, perhaps, also to the study of the "normal" submicroscopic particles.