Mouse mammary cells ind-valine medium

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Abstract
Cells of a mouse mammary epithelial cell line as well as fibroblasts from a mouse mammary explant were severely inhibited from proliferating in a medium in whichd-valine was substituted forl-valine. After the first few days ind-valine medium, the number of epithelial cells did not increase despite the fact that a few percent continued to synthesize DNA. The cells did recognize the presence of thed-valine in the medium because cells ind-valine increased in volume and their numbers remained stationary, whereas cells without valine shrank and the cell numbers decreased with time.

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