Microinjection of DNA into Aedes Triseriatus Ova and Detection of Integration
- 1 November 1988
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 39 (5) , 502-510
- https://doi.org/10.4269/ajtmh.1988.39.502
Abstract
A technique was developed for microinjection and transformation of Aedes triseriatus mosquitoes. Mosquito ova can be readily microinjected with approximately 0.9 nl of inoculum if desiccated and inoculated before hardening of the chorion. Ova were injected with molecular constructs that conferred antibiotic (G418) resistance to larvae. Insertion of the antibiotic resistance construct into the mosquito genome was demonstrated by Southern blot analysis.This publication has 14 references indexed in Scilit:
- Delay of Disease Development in Transgenic Plants That Express the Tobacco Mosaic Virus Coat Protein GeneScience, 1986
- Identification and immunochemical analysis of biologically active Drosophila P element transposaseCell, 1986
- Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicingCell, 1986
- Evolution of Bunyaviruses by Genome Reassortment in Dually Infected Mosquitoes ( Aedes triseriatus )Science, 1985
- Fate of DNA injected into early Drosophila embryosDevelopmental Biology, 1985
- A transposable P vector that confers selectable G418 resistance to Drosophila larvaeThe EMBO Journal, 1985
- A new dominant hybrid selective marker for higher eukaryotic cellsJournal of Molecular Biology, 1981
- Molecular Basis of Bunyavirus Transmission by Mosquitoes: Role of the Middle-Sized RNA SegmentScience, 1981
- Expression of a transposable antibiotic resistance element in SaccharomycesNature, 1980
- Venereal Transmission of La Crosse Virus from Male to Female Aedes TriseriatusThe American Journal of Tropical Medicine and Hygiene, 1978