Identification of an essential glutamate residue in the active site of endoglucanase III from Trichoderma reesei
Open Access
- 25 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 316 (2) , 137-140
- https://doi.org/10.1016/0014-5793(93)81202-b
Abstract
n‐Propyl, n‐butyl and n‐pentyl β‐cellobiosides with a reactive ω‐epoxide in their aglycon completely and irreversibly inactivate endoglucanase III from Trichoderma reesei. The pentyl derivative was found to be most effective. From these affinity labeling experiments evidence was found for the implication of Glu329 in the reaction mechanism. This is discussed in relation to other structural/functional data known for endoglucanase III and several other family A glycanases.Keywords
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