An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR
- 1 October 1997
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 8 (2) , 135-137
- https://doi.org/10.1007/bf02752257
Abstract
A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65°C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.Keywords
This publication has 6 references indexed in Scilit:
- Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.Proceedings of the National Academy of Sciences, 1990
- Quantitation of mRNA by the polymerase chain reaction.Proceedings of the National Academy of Sciences, 1989
- Access to a Messenger RNA Sequence or Its Protein Product Is Not Limited by Tissue or Species SpecificityScience, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseScience, 1988
- Shotgun DNA sequencing using cloned DNase I-generated fragmentsNucleic Acids Research, 1981