Comparison of Effectiveness of Various Proteolytic Enzymes in Blood‐Group Antibody Detection

Publisher Website
Google Scholar
Abstract
The present project was undertaken to compare the activity of enzyme preparations currently used; these are the six described in Technical Methods and Procedures of the American Association of Blood Banks, 4th Edition, 1966, three commercially available stabilized enzyme solutions, and one commercially prepared experimental bromelin solution. The latter four were tested according to instructions of the manufacturers. An antibody and an appropriate cell suspension were tested with each of the ten enzyme systems at one time. Antibodies studied were related to several blood group systems; cold agglutinins and, where possible, several examples of each specificity were included. Certain antibodies were detected in routine testing with enzyme‐treated cells, although this was contrary to some previously published reports.