Separation of Two Salmonella O-Antigen Polysaccharides by Means of a Glutaraldehyde-Concanavalin-A Polymer. Primary Studies on the Separated Fractions

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Abstract
The S. zuerich (1,9,12,(46),27) cell wall O-polysaccharides were characterized as a mixture of 2 immunologically distinct fractions differing in the presence or absence of the antigenic determinant 1 usually carried by side chains of an .alpha.-D-glucosyl residue. Concanavalin A was used in separating the 2 O-polysaccharides. The procedure involves polymerization of concanavalin A with glutaraldehyde, followed by selective adsorption of the polysaccharide containing O factor 1 and elution with D-glucose. Immunological and chemical analyses showed that the separation was successful and demonstrated chemical differences between these 2 fractions in relation to their immunological behavior.