A MULTIPLE-COLUMN HYDRAULIC METHOD FOR FRACTIONATION OF PARTICLES FOR CHROMATOGRAPHIC COLUMNS

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Abstract
An apparatus for fractionation of particles is described. Liquid at constant temperature is freed of air bubbles and circulated at constant flow rate through a series of vertical columns of progressively increasing diameters, a settling bottle, and a filter. The particles to be fractionated are either added gradually to the first column by passage of the return flow of liquid through a particle reservoir or are added at the outset to the first column. The flow of liquid distributes the particles between the columns according to particle size and density. Illustrative data are given for fractionation of a cross-linked dextran, a diatomaceous earth, a microbead ion-exchange resin, and a micropowder ion-exchange resin.