THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS

1 May 1987

journal article
research article
Published by Wiley in Photochemistry and Photobiology

Vol. 45 (S1) , 781-786
https://doi.org/10.1111/j.1751-1097.1987.tb07882.x

Abstract

A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.

This publication has 18 references indexed in Scilit:

ACTION SPECTRA FOR THE INDUCTION OF PYRIMIDINE(6‐4)PYRIMIDONE PHOTOPRODUCTS AND CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL HUMAN SKIN FIBROBLASTS
Photochemistry and Photobiology, 1987
The relative cytotoxicity and mutagenicity of cyclobutane pyrimidine dimers and 〈6—4〉 photoproducts in escherichia coli cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1986
Action Spectrum for the Formation of Endonuclease-sensitive Sites and (6-4) Photoproducts Induced in a DNA Fragment by Ultraviolet Radiation
International Journal of Radiation Biology, 1986
(6–4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers
Mutation Research Letters, 1985
INDUCTION OF PHOTOPRODUCTS IN SYNTHETIC POLYNUCLEOTIDES BY FAR AND NEAR ULTRAVIOLET RADIATION
Photochemistry and Photobiology, 1984
MUTATION INDUCTION BY AND MUTATIONAL INTERACTION BETWEEN MONOCHROMATIC WAVELENGTH RADIATIONS IN THE NEAR‐ULTRAVIOLET AND VISIBLE RANGES
Photochemistry and Photobiology, 1980
Mutation to ouabain-resistance in Chinese hamster cells: Induction by ethyl methanesulphonate and lack of induction by ionising radiation
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
Amplification and characterization of a β-globin gene synthesized in vitro
Cell, 1976
A comparison of the 8-azaguanine and ouabain-resistance systems for the selection of induced mutant Chinese hamster cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
NEAR‐U.V. PHOTOLYSIS OF PYRIMIDINE HETEROADDUCTS IN E. COLI DNA*
Photochemistry and Photobiology, 1970