CHEMICAL STUDIES ON CELLULAR COMPONENTS OFBORDETELLA PERTUSSISI

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Abstract
Onoue, Kaoru, (Kyushu University, Fukuoka, Japan),Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components ofBordetella pertussis. I. Purification and properties of agglutinogen. J. Bacteriol.82:648–656. 1961.—A method is described for the preparation of the agglutinogen ofHemophilus pertussisin phase I by methanol precipitation and ion-exchange chromatography.High homogeneity of this agglutinogen was evidenced by chromatography, but a trace of impurity was detected by ultracentrifugal analysis and by an agar gel diffusion test.The agglutinogen produced an allergic skin reaction in rabbits which had been immunized with whole phase I organisms.Chemical studies indicate that the agglutinogen should be a simple protein in nature. The approximate molecular weight was determined to be 10,000 by Archibald's method.