The extracellular metalloprotease ofSerratia marcescens: I. Purification and characterization
- 1 November 1976
- journal article
- conference paper
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 13 (2) , 95-100
- https://doi.org/10.1007/bf01837059
Abstract
An extracellular protease ofSerratia marcescens produced during growth on skim milk medium was isolated by ethanol precipitation. The protease was purified by salt fractionation, DEAE-cellulose ion exchange chromatography and gel filtration chromatography on Agarose P-100. It has a broad optimum from pH 6.0 to 9.0 and a temperature optimum of 45°C for proteolytic activity on casein. It was classified as a metallo-protease by virtue of its inactivation by metal-ion chelators and reactivation by ferrous ions. Proteolytic activity was not affected by diiso-propylfluorophosphate, p-chloromercuribenzoate and dithiothreitol.This publication has 21 references indexed in Scilit:
- Bacillus megaterium neutral protease, a zinc-containing metalloenzymeBiochimica et Biophysica Acta (BBA) - Protein Structure, 1971
- Serratia ProteaseAgricultural and Biological Chemistry, 1971
- Proteases of the genus Bacillus. I. Neutral proteasesBiotechnology & Bioengineering, 1970
- [40] Extracellular proteinase from Penicillium notatumPublished by Elsevier ,1970
- Biochemical and physiological properties of an extracellular protease produced byBacillus megateriurnBiotechnology & Bioengineering, 1969
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Dithiothreitol, a New Protective Reagent for SH Groups*Biochemistry, 1964
- SOME CHARACTERISTICS OF PROTEINASES OF AN OBLIGATELY PSYCHROPHILIC RED-PIGMENTED BACTERIUM AND OF SERRATIA MARCESCENSCanadian Journal of Microbiology, 1963
- PROTEOLYTIC ENZYMESAnnual Review of Biochemistry, 1960
- STUDIES ON THE BIOSYNTHESIS OF EXTRACELLULAR PROTEASES BY BACTERIAThe Journal of general physiology, 1956