EFFECT OF SEPARATING BULL SEMEN INTO X-CHROMOSOME-BEARING AND Y-CHROMOSOME-BEARING FRACTIONS ON THE SEX-RATIO OF RESULTING EMBRYOS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 48  (3) , 294-298
Abstract
Day 12 to day 15 bovine embryos (76), collected from 14 donors which were inseminated with either X or Y chromosome-bearing spermatozoa fractions of semen separated by a thermal convection counterstreaming sedimentation and forced convection galvanization process, were processed for sexing by chromosomal analysis. Embryos (57) were sexed; 20 from Y chromosome-bearing and 37 from X chromosome-bearing fractions of semen. Statistical analysis of the sexing data indicated that there was no significant difference in the male: female ratio for donors receiving male fractions compared to those receiving female fractions. The Y chromosome-bearing fractions produced a male:female ratio that was indistinghishable from the expected 1:1 ratio. The X chromosome-bearing fractions of semen produced a highly significant deviation from the expected 1:1 ratio towards the male.

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