Gibberellin A4Production bySphaceloma manihoticola,Causal Agent of Cassava Superelongation Disease

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Abstract
Symptoms of superelongation disease of cassava (caused by S. manihoticola), include striking internode elongation, suggesting a hormonal role in the disease. Gibberellin A4 was purified from culture filtrate of pathogenic isolates by base-acid partitioning with ethyl acetate followed by silica gel column chromatography. Biologically active fractions were identified by lettuce seedling hypocotyl elongation bioassay. The fungal product was identified as gibberellin A4 by combined GLC and mass spectrometry. Treatment of plants with known gibberellin A4 and that purified from pathogen filtrate reproduced secondary symptoms.