Stereocontrolled Synthesis of Dithymidine Phosphorothioates by Use of a Functionalized 5'- Protecting Group Bearing an Imidazole Residue

Abstract
Diastereoselective formation of internucleotidic phosphorothioate triester bonds was achieved by use of 3-(imidazol-1-ylmethyl)-4′,4″-dimethoxytrityl (IDTr) as a 5′-hydroxyl protecting group in the phosphotriester approach. After removal of all the protecting groups, stereochemistry of the major product was determined as the Spconfiguration by enzymatic digestion.

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