Generation of calcium waves in living cells by pulsed-laser-induced photodisruption
- 20 August 2001
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 79 (8) , 1208-1210
- https://doi.org/10.1063/1.1397255
Abstract
Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca 2+ waves were induced by femtosecond pulsed-laserillumination. Living HeLa cells were exposed to focused 140 fs pulses of 780 nm wavelength at 30 mW average power. Ca 2+ waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell. Photoinduced generation of Ca 2+ waves can be performed at any point inside the cell, an improvement over previous mechanical or biochemical stimulation techniques.Keywords
This publication has 9 references indexed in Scilit:
- Three-dimensional subsurface microprocessing of collagen by ultrashort laser pulsesApplied Physics Letters, 2001
- Calcium - a life and death signalNature, 1998
- Femtosecond Optical Breakdown in DielectricsPhysical Review Letters, 1998
- Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulinNature, 1997
- Cell-to-cell spread of calcium signals mediated by ATP receptors in mast cellsNature, 1992
- All‐or‐nothing Ca2+ mobilization from the intracellular stores of single histamine‐stimulated HeLa cells.The Journal of Physiology, 1992
- Mechanical stimulation and intercellular communication increases intracellular Ca2+ in epithelial cells.Cell Regulation, 1990
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990
- Effect of Electrical Stimulation on Uptake and Release of Calcium by the Endoplasmic ReticulumNature, 1965